For this experiment, photosynthetic pigments were separated through paper chromatography and the absorption spectra of these were measured through absorbance spectrophotometry pigment extraction old and young leaf samples were obtained around the up campus each set of samples was shredded into smaller pieces and weighed to twenty grams. The plastids contain different pigments that give the leaf its color the pigment located in the chloroplasts is the one we see in most leaves most of the time this pigment is chlorophyll, which is green but leaves contain other pigments, and they are located in the chromoplasts. All plants have in common the photosynthetic pigment of chlorophyll, but the other accessory pigments as different these accessory pigments can be the same between two red algae, or two green algae. Pigments are then identified by the colours and the rfvalues obtained by comparing the rf value from table 1 and table 2, we can find that there is carotene, phaeophytin, xanythophyll, chlorophyll a and chlorophyll b present in the spinach leaves. Xanthophylls are yellow pigments, and carotenoids give leaves an orange color photosynthesis also uses these pigments during the summer, but chlorophyll, a stronger pigment, overpowers them.
The photosynthetic pigment is used to absorb light during the process of photosynthesis there are five pigments usually found in spinach leaves: carotene (yellow), phaeophytin (yellow-grey), xanthophyll (yellow-brown), chlorophyll a (blue-green), chlorophyll b(green) photosynthetic pigment is located in the chloroplast of the leaf. After 3-5 minutes, the distance traveled by each pigment and solvent were measured plants extracted| color/ pigment| rf values| kangkong| yellow (solvent)| 0 9 mm| | brown (solute)| 0 08 mm| purple leaves| green (solvent)| 0 58 mm| | purple (solute)| 0 13 mm| golden bush| green (solvent)| 0 6 mm| | brown (solute)| 0 067 mm| table 1: pigments and rf values for each plant extract the distance traveled relative to the solvent is called the rf value, or the retardation value. For example the kangkong leaf also contains different pigments even though the leaf is dominated by the color green we have observed that the kangkong leaf not only carries a green pigment, but that it also carries yellow and brown pigment through the chromatography literature cited mcmurry, john 2010.
Since chlorophyll is such a dominant pigment in green plants, this domination hides the color of the carotenes and xanthophylls in the leaves we will write a custom essay sample on separation of photosynthetic pigments by paer chromatography specifically for you.
All plants that photosynthesize contains chlorophyll a, while, chlorophyll b is a second kind of chlorophyll that occurs only in green algae plants accessory pigments absorb light energy and transfers energy to chlorophyll a during photosynthesis two accessory pigments are carotenes and xanthophylls. Tittle: separation of leaves pigment introduction the photosynthetic pigment is used to absorb light during the process of photosynthesis there are five pigments usually found in spinach leaves: carotene (yellow), phaeophytin (yellow-grey), xanthophyll (yellow-brown), chlorophyll a (blue-green), chlorophyll b(green. This primarily uses a pigment that reflects green light, which gives the leaves their characteristic color in autumn, when colder, shorter days arrive, many kinds of trees no longer make food energy with their leaves and, consequently, no longer need the green pigment.
The point of this experiment is to look at the polarity of some of the common pigments in plant leaves and how that polarity affects their interactions with the cellulose fibers in paper and a few solvents and to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts. Conclusion after completing this lab, each pigment present was separated and identified based on its solubility to the solvent and the r f value of each pigment was determined based on their distance traveled. Chromatography of photosynthetic pigments abstract in this experiment a process of chromatography was used to separate chlorophyll a, chlorophyll b, xanthophyll, and beta carotene when these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength introduction photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy.
I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin discussion questions: 1 discussion questions: 1. Because the accessory pigments seemed to be important in the well-being of a plant, i believed that the leaves from both the deciduous and the broadleaf evergreens would contain all the pigments: chlorophyll, xanthophylls, carotenoids, and anthocyanins.
Algal pigments essay examples - algal pigments all photosynthesizing plants have certain pigments in them by which they capture sunlight all plants have in common the photosynthetic pigment of chlorophyll, but the other accessory pigments as different.
Essay on separation of leaves pigment tittle: separation of leaves pigment introduction the photosynthetic pigment is used to absorb light during the process of photosynthesis.